Ndị ọrụ nyocha ahụ jiri pipeline na metagenomics ọhụrụ dị elu-throughput kwụsiri ike isotope probing (HT-SIP) iji nweta nlele mbụ na microbiome na-arụ ọrụ nke gbara gburugburu Symbiont osisi bara uru, fungi mycorrhizal arbuscular (AMF). Ebe E Si Nweta: Lawrence Livermore National Laboratory
Ijikọta njirimara nke ụmụ nje ndị dị n'ọhịa na àgwà physiological ha na ọrụ gburugburu ebe obibi bụ isi ebumnuche maka microbiologists gburugburu ebe obibi. N'ime usoro ndị na-agba mbọ maka ebumnuche a, a na-ahụta Stable Isotope Probing-SIP dị ka ihe kachasị dị irè maka ịmụ ihe microorganisms na-arụ ọrụ na ntọala okike.
Ndị ọkà mmụta sayensị Lawrence Livermore National Laboratory (LLNL) emepụtala usoro ọhụrụ - SIP dị elu - nke na-eme ka usoro dị iche iche dị na usoro nyocha nke isotope kwụsiri ike, na-enye ohere nyocha nke ọrụ microbial nke microorganisms n'okpuru ọnọdụ ezi uche, na-enweghị mkpa nke ụlọ nyocha.
Na SIP, a na-amata ụmụ nje ndị na-arụ ọrụ site na ntinye nke isotopes kwụsiri ike n'ime biomass ha. Ọ bụ otu n'ime ụzọ kachasị ike na microbial ecology ebe ọ nwere ike ịchọpụta microbes na-arụ ọrụ na àgwà ha physiological (eji mkpụrụ osisi, biochemistry cellular, metabolism, uto, ọnwụ) na obodo mgbagwoju anya n'okpuru ọnọdụ obodo.
Dịka, usoro SIP na-achọ nnukwu aka na-arụ ọrụ ma na-enye ohere maka ọnụ ọgụgụ dị nta nke nlele. Mana usoro LLNL ọhụrụ ahụ chọrọ otu ụzọ n'ụzọ isii nke ọnụ ọgụgụ aka na-arụ ọrụ ma e jiri ya tụnyere akwụkwọ ntuziaka SIP ma na-enye ohere ka ahazi sample 16 n'otu oge.
"Usoro anyị nwere obere akpaaka na-ebelata oge ndị ọrụ ma na-emezigharị ọmụmụ site na ịchụso usoro SIP kachasị arụ ọrụ," onye ọkà mmụta sayensị LLNL Erin Nuccio kwuru, na onye ndu ode akwụkwọ na-apụta na akwụkwọ akụkọ Microbiome. "Ugbu a, anyị ejirila usoro a hazie ihe karịrị otu puku sample, gụnyere ụfọdụ sitere na microhabitat ala na-amụchaghị akwụkwọ."
Otu microhabitat dị otú ahụ bụ ala gbara gburugburu anụ ahụ nke mycorrhizae ozugbo - ụdị fungi nke na-emepụta mmekọrịta symbiotic na 72% nke osisi ala niile. Na mgbanwe maka carbon osisi, ero (arbuscular mycorrhizal fungi) na-enye ndị ọbịa ya ihe ndị dị mkpa dị ka nitrogen, phosphorus na mmiri.
N'ime ihe omumu ihe omuma nke a, ndi odee gosiputara "web nri" nke mmekorita nke mycorrhizal fungi mere n'ime ala.
"Anyị chere na nke a bụ ụzọ bụ isi maka ka carbon osisi si ekesa n'ime ala. Ala na-ejide ọdọ mmiri kachasị ukwuu nke carbon organic na-arụsi ọrụ ike na mbara ala, "onye na-emekọ ihe ọnụ bụ Jennifer Pett-Ridge, onye bụ onye ndu oru ngo LLNL na onye isi nke Department of Energy Office of Science "Microbes Persist" Ala Microbiome Scientific Focus Area . "Anyị na-ahazi ntakịrị DNA, chọpụta ihe ndị dị ndụ na-arụ ọrụ wee wughachi mkpụrụ ndụ ihe nketa na mmekọrịta ha nwere."
Ndị edemede LLNL ndị ọzọ gụnyere Steven Blazewicz, Marissa Lafler, Ashley Campbell, Jeffrey Kimbrel, Jessica Wollard, Rachel Hestrin yana ndị nyocha sitere na Lawrence Berkeley National Laboratory, DOE Joint Genome Institute na Mahadum California, Berkeley.